CAS:110623-72-8,朝藿定A廠家
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應(yīng)用領(lǐng)域: 僅供科研使用,不得用于醫(yī)學(xué)診斷。使用前仔細閱讀本說明書。
CAS:110623-72-8,朝藿定A廠家 產(chǎn)品均附有使用說明書,質(zhì)量要求,期和裝量
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美國國家藥典USP標(biāo)準物質(zhì)、歐洲藥典EP標(biāo)準物質(zhì),歐洲標(biāo)準局標(biāo)準品irmm/ERM——中國區(qū)特約代理,產(chǎn)品齊全,所有標(biāo)準品均可訂購,價格便宜。
CAS:110623-72-8,朝藿定A廠家 工作時間內(nèi)免費的技術(shù)咨詢和指導(dǎo)
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對苯二胺硫酸鹽 Cas號:106261-49-8 試劑夏季促銷中
對苯二胺硫酸鹽 Cas號:106261-49-8 試劑價格:聯(lián)系客服為您查詢促銷后價格!國產(chǎn)l進口均可提供,歡迎訂購。。
對苯二胺硫酸鹽 Cas號:106261-49-8 庫存狀態(tài):現(xiàn)貨充足。全國各地快遞免費送貨上門(S)-3-氨基-4-苯基丁酸鹽酸鹽Cas號:448245-52-1H-Sar-NH2HClCas號:179075-88-8肌氨酸乙酯鹽酸鹽Cas號:445473-58-5Cbz-L-精氨酸鹽酸鹽Cas號:344790-87-04-甲基-5-羥甲基咪唑鹽酸鹽Cas號:32372-82-01-丁基-3-甲基咪唑鹽酸鹽 Cas號:108412-04-0
對苯二胺硫酸鹽 Cas號:106261-49-8
Icaritin | 淫羊藿素 | 5240-95-9 | 98% |
Jatrorrhizine Hydrochloride | 鹽酸藥根堿 | 960383-96-4 | 98% |
JujubosideA | 酸棗仁皂苷A | 55466-04-1 | 98% |
Jujuboside B | 酸棗仁皂苷B | 55466-05-2 | 98% |
Koumine | 鉤吻素子 | 1358-76-5 | 98% |
L-4-Hydroxyisoleucine | L-4羥基異亮氨酸 | 6001-78-8 | 98% |
Liquiritigenin | 甘草素 | 578-86-9 | 98% |
Luteoloside | 木犀草苷 | 5373-11-5 | 98% |
Levistilide A | 歐當(dāng)歸內(nèi)酯A | 88182-33-6 | 98% |
KH-E10621 | 人庚型肝炎病毒IgM(HGV-IgM)elisa試劑盒 |
96T/48T產(chǎn)品資料:
檢測范圍: 96T
12ng/L-480ng/L
應(yīng)用領(lǐng)域:
本試劑盒用于測定人血清、血漿及相關(guān)液體樣本中腫瘤壞死因子α(TNF-α)含量。
工作原理:
本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中人腫瘤壞死因子α(TNF-α)水平。用純化的人腫瘤壞死因子α(TNF-α)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入腫瘤壞死因子α(TNF-α),再與HRP標(biāo)記的腫瘤壞死因子α(TNF-α)抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的腫瘤壞死因子α(TNF-α)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準曲線計算樣品中人腫瘤壞死因子α(TNF-α)濃度。
KH-E10001 人原鈣黏素1(PCDH1)elisa試劑盒 96T/48T 試劑盒組成
1 | 30倍濃縮洗滌液 | 20ml×1瓶 | 7 | 終止液 | 6ml×1瓶 |
2 | 酶標(biāo)試劑 | 6ml×1瓶 | 8 | 標(biāo)準品(720ng/L) | 0.5ml×1瓶 |
3 | 酶標(biāo)包被板 | 12孔×8條 | 9 | 標(biāo)準品稀釋液 | 1.5ml×1瓶 |
4 | 樣品稀釋液 | 6ml×1瓶 | 10 | 說明書 | 1份 |
5 | 顯色劑A液 | 6ml×1瓶 | 11 | 封板膜 | 2張 |
6 | 顯色劑B液 | 6ml×1/瓶 | 12 | 密封袋 | 1個 |
標(biāo)本要求
1.標(biāo)本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
KH-E10001 人原鈣黏素1(PCDH1)elisa試劑盒 96T/48T 操作流程:
1. 標(biāo)準品的稀釋:本試劑盒提供原倍標(biāo)準品一支,用戶可按照下列圖表在小試管中進行稀釋。
360ng/L | 5號標(biāo)準品 | 150μl的原倍標(biāo)準品加入150μl標(biāo)準品稀釋液 |
180ng/L | 4號標(biāo)準品 | 150μl的5號標(biāo)準品加入150μl標(biāo)準品稀釋液 |
90ng/L | 3號標(biāo)準品 | 150μl的4號標(biāo)準品加入150μl標(biāo)準品稀釋液 |
45ng/L | 2號標(biāo)準品 | 150μl的3號標(biāo)準品加入150μl標(biāo)準品稀釋液 |
22.5ng/L | 1號標(biāo)準品 | 150μl的2號標(biāo)準品加入150μl標(biāo)準品稀釋液 |
2. 加樣:分別設(shè)空白孔(空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、標(biāo)準孔、待測樣品孔。在酶標(biāo)包被板上標(biāo)準品準確加樣50μl,待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl(樣品最終稀釋度為5倍)。加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻。
3. 溫育:用封板膜封板后置37℃溫育30分鐘。
4. 配液:將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用
5. 洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。
6. 加酶:每孔加入酶標(biāo)試劑50μl,空白孔除外。
7. 溫育:操作同3。
8. 洗滌:操作同5。
9. 顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.
10. 終止:每孔加終止液50μl,終止反應(yīng)(此時藍色立轉(zhuǎn)黃色)。
11. 測定:以空白空調(diào)零,450nm波長依序測量各孔的吸光度(OD值)。 測定應(yīng)在加終止液后15分鐘以內(nèi)進行。
操作程序總結(jié):
計算
以標(biāo)準物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),在坐標(biāo)紙上繪出標(biāo)準曲線,根據(jù)樣品的OD值由標(biāo)準曲線查出相應(yīng)的濃度;再乘以稀釋倍數(shù);或用標(biāo)準物的濃度與OD值計算出標(biāo)準曲線的直線回歸方程式,將樣品的OD值代入方程式,計算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實際濃度。
KH-E10001 人原鈣黏素1(PCDH1)elisa試劑盒 96T/48T 注意事項:
1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2.濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。
3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差。一次加樣時間最好控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時做標(biāo)準曲線,最好做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準品孔第一孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請最后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6.底物請避光保存。
7.嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準.
8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9.本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準。
保存條件及有效期
1.試劑盒保存:;2-8℃。
2.有效期:6個月
公司介紹: 上海森雅生物技術(shù)有限公司是一家專業(yè)經(jīng)營生物試劑、儀器耗材的公司,經(jīng)過多年不懈的努力,已成為國內(nèi)生命科學(xué)領(lǐng)域產(chǎn)品的主要供應(yīng)商之一,自主研發(fā)的elisa試劑盒更是具有國內(nèi)頂尖的技術(shù)水平。公司的服務(wù)和業(yè)務(wù)網(wǎng)絡(luò)遍及全國,在同行獲得一致好評。 特別是生物試劑和細胞產(chǎn)品更是種類齊全、價格實惠,美國原裝進口原代細胞,專業(yè)培養(yǎng)基,常用傳統(tǒng)培養(yǎng)基,細胞培養(yǎng)用的相關(guān)試劑,對照品標(biāo)準品,科研抗體抗原,生化試劑產(chǎn)品品種齊全,試劑耗材種類繁多。并代理國內(nèi)外許多著名品牌等等。 公司的規(guī)模和產(chǎn)品種類日益擴大和更新,我們一直秉承著代理優(yōu)質(zhì)品牌的產(chǎn)品,服務(wù)好每一位顧客,將最新的科學(xué)技術(shù)和方法推薦顧客的服務(wù)宗旨為廣大客戶提供各類服務(wù),產(chǎn)品、技術(shù)咨詢、國際國內(nèi)信息,每年組織大批員工出國學(xué)習(xí),掌握國際先進的技術(shù)和信息。 公司的宗旨是:為客戶提供質(zhì)量的產(chǎn)品,的技術(shù)服務(wù)!
4-[(4-甲基哌嗪-1-基)甲基]苯甲酸二鹽酸鹽,106261-49-8,4-[(4-methylpiperazin-1-yl)methyl]benzoic acid dihydrochloride 0.5 hydrate 更多產(chǎn)品請進入本公司官方網(wǎng)站查看 http://www.ziyi-reagent.com/ | ||||||||||||||||||||||||||
4-[(4-methylpiperazin-1-yl)methyl]benzoic acid dihydrochloride 0.5 hydrate | ||||||||||||||||||||||||||
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豬皮質(zhì)類固醇結(jié)合球蛋白(SERPINA6) ELISA kit 英文名稱: Pig Corticosteroid-binding globulin(SERPINA6) ELISA kit 別名: CBG, corticosteroid binding globulin|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6|transcortin 貨號: CSB-EL021062PI 中文價格: 4200 規(guī)格: 96T 種屬: Pig 待測物名稱: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6 縮寫: SERPINA6 蛋白功能1: Transport 蛋白功能3: Transport 檢測范圍: Request Information 靈敏度: Request Information 反應(yīng)時間: 1-5h 所需樣本體積: 50-100ul 檢測波長: 450 nm 下載: 注:產(chǎn)品說明書可能有改進,請直接與CUSABIO產(chǎn)品專員聯(lián)系,索取最新版說明書! 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA6 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特異性: This assay has high sensitivity and excellent specificity for detection of Pig SERPINA6. No significant cross-reactivity or interference between Pig SERPINA6 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. 樣本搜集及儲存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 檢測步驟: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 結(jié)果計算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the SERPINA6 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.